14T MRI/MRS/NMR at SLU
HIGH RESOLUTION IMAGING AND SPECTROSCOPY CORE FACILITY
14 Tesla (600 MHz) High Resolution IMaging (HRIM)
The High Resolution Nuclear Magnetic Resonance (NMR) Spectroscopy, Magnetic Resonance Imaging (MRI), and Magnetic Resonance Spectroscopy (MRS) Core Facility at the South Lake Union campus assists researchers from the University of Washington Mitochondria and Metabolism Center and the research community at large.
The facility is capable of multi-nuclear, high resolution NMR as well as in vivo MRS and micro MRI. All projects are welcome.
Facility
The cornerstone of the facility is a state-of-the-art 14 Tesla (600 MHz) vertical wide bore Bruker Magnetic Resonance Spectrometer. The magnet system is fully equipped for NMR and provides cost effective in vivo MRI and MRS in small animals, such as mice.
It is equipped with a sample temperature control unit and a biological sensor system directly linked to the instrument, allowing animal monitoring and control of studies. The laboratory has a satellite workstation for data processing, a lab bench area for sample/animal preparation, an isoflurane anesthesia system for in-vivo work, and a small refrigerator for temporary storage of samples and reagents.
The facility is currently approved for non-survival and survival work at the BSL-1 and BSL-2 levels and can be added to researchers’ protocols at these levels. Please review our Lab Safety Resources page to find links to the UW IACUC and their required forms.
Equipment and Software
High Resolution Spectroscopy
Software: TOPSPIN (Bruker-Biospin)
| High Resolution Probes | ||
| Type | Tuning Range | Variable Temperature Range |
| 3 mm BBO | 15N – 31P with 1H decoupling | -120 to 150 °C |
| 5 mm BBO | 15N to 31P with 1H decoupling | -150 to 180 °C |
| 10 mm BBO | 109Ag to 31P with 1H decoupling | -130 to 150 °C |
| 20 mm BBO | 109Ag to 31P with 1H decoupling | |
Imaging
Software: ParaVision (Bruker-Biospin)
| Micro-AHS/RF2.5 In Vivo 25 mm ID micro-imaging probe with: |
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| MicroMouse 2.5-In Vivo 30 mm ID micro-imaging probe |
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